Expression of a fusion protein of Pyrus pyrifolia S-RNase with glutathione-S-transferase in E. coli


Niranjali Gamage2, Takanori Matsuura1, Naoko Norioka1, Yumi Yoshimura1, Takeshi Takasaki2, Tetsu Nakanishi2, Shigemi Norioka1

1Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan
2Faculty of Agriculture, Kobe University, Kobe, Hyogo 657-8501, Japan

Biotechnology Letters 22(17) 1413-1417 (2000)

S-RNase is a style-specific ribonuclease which is associated with gametophytic self-incompatibility. An expression vector of a fusion protein of Pyrus pyrifolia (Japanese pear) S3-RNase with glutathione-S-transferase (GST) was constructed and transformed into E. coli. Using this system, the fusion protein, GST-S3-RNase, was expressed as an active form and can be used for screening pollen S-gene product(s).




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