Purification and crystallization of Japanese pear S-RNase associated with gametophytic self-incompatibility


Takanori Matsuuraa, Masaki Unnob, Hiroaki Sakaib, Tomitake Tsukiharab and Shigemi Noriokaa

aDivision of Protein Chemistry, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan,
bDivision of Protein Crystallography, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan

Acta Crystallographica D57(1), 172-173 (2001)

S-RNase is a 25 kDa pistil-specific protein associated with gametophytic self-incompatibility. The S-RNase is secreted into the transmitting tissue of the pistil and is responsible for the discrimination of pollen S-alleles. Crystals of Japanese pear S3-RNase were obtained by the hanging-drop vapour-diffusion method. Preliminary X-ray data showed that the crystals diffract to a 1.5 Å resolution and belong to the space group P21, with unit-cell parameters a = 45.4, b = 52.4, c = 47.4 Å, α = γ = 90, β = 106.5°.




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