Crystallization and preliminary X-ray crystallographic study of disproportionating enzyme from potato


Kayo Imamuraa, Takanori Matsuurab, Zhengmao Yea, Takeshi Takahac, Kazutoshi Fujiic, Masami Kusunokib and Yasunori Nittaa

aLaboratory of Enzyme Chemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan,
bInstitute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan,
cBiochemical Research Laboratory, Ezaki Glico Co. Ltd, Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan

Acta Crystallographica F61(1), 109-111 (2005)

Disproportionating enzyme (D-enzyme; EC 2.4.1.25) is a 59 kDa protein that belongs to the α-amylase family. D-enzyme catalyses intramolecular and intermolecular transglycosylation reactions of α-1,4 glucan. A crystal of the D-enzyme from potato was obtained by the hanging-drop vapour-diffusion method. Preliminary X-ray data showed that the crystal diffracts to 2.0 Å resolution and belongs to space group C2221, with unit-cell parameters a = 69.7, b = 120.3, c = 174.2 Å.




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